IF analysis of GAL4 using anti-GAL4 antibody (PB9952).
GAL4 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The celss were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-GAL4 Antibody (PB9952) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Flow Cytometry analysis of THP-1 cells using anti-GAL4 antibody (PB9952).
Overlay histogram showing THP-1 cells stained with PB9952 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GAL4 Antibody (PB9952,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.